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We produce all possible Heating and Cooling Stages for microscopy and spectroscopy
We produce all possible Microscopic Systems
3D inspection systems, Industrial quality control, Material analysis, Medical and pharmaceutical high throughput screening, Research and Development test systems and many more. We can build hybrid systems, combining Microscopy with Spectroscopy. Confocal and super resolution. We provide all sorts of motorized stands and robotics for high throughput microscopy. New in our portfolio: The 3D Maker, turning any microscope into a high end 3D system.
3D Digital Video Microscopy:
Build Your Own System!
A vast field of applications
The field of applications in microscopy has become incredible vast. Ever since digital camera’s, advanced optics, sensors, stepper motors and related software came into play, the field of applications has expanded to such an extent that it takes more than a full time job to cope with all the nuances of technologies which are now at hand for the user.
3D Digital Microscopy
The evolution of digital video microscopy has been very fast considering the amount of systems which are now available on the market. For a few hundred of Euro’s one can buy a hand held microscope nowadays which provides good quality images. With the rise of microscopy building blocks, which can be bought off the shelve, one can now build your own system (BYOS) for prices which are significantly less than the high end systems. The quality of these BYOS systems are superb. Pricewise these BYOS systems are becoming more and more in reach for any user.
An example of a BYOS Digital Video Microscope
The stand and accessories
One aspect in the choice of building your own 3D system is often neglected. This aspect is as important as all the other components needed to build a 3D microscope. The stand on which the microscope is constructed must be very rugged and must have a mechanism to lift the optics in precise steps in a vertical direction. For that purpose there are complete systems in the market which allows a lens to be lifted in steps less than 1 µm. Such a stand has a base plate with illumination (for bright field 3D, or if needed for 3D fluorescence analysis), with a vertical bar and a spindle motorized slide powered with a high quality stepper motor.
A motorized stand with accessories
A high quality lenses
There are many suitable microscopy lenses in the market which are produced by popular lens manufacturers. Any quality lens in this respect can be used which has a certain depth of field and field of view needed for the 3D application. Typical depth of field varies between 2 mm to 10 µm. Typical Field of view varies between 2 mm to 10 µm.
A typical high quality microscopic lens
A good quality camera with a suitable format fitting the lens, with appropriate software is required to make a 3D microscopic system. There quite good camera’s in the market which can be used for this purpose. Typical parameters are: USB2/3,10 MP, pixel size 1.7 µm.
A digital camera
For applying 3D microscopy illumination can be provided either through back-light, ring light or coaxial. All of these accessories are available in the market.
Once all the modules are assembled, stand, lens, camera and illumination, then the automation needed to generate 3D images is accomplished through a controller which can control the camera, stepper motor and illumination. Such controllers are available in the market.
A typical 3D controller
Software which controls the whole system is the final module which is needed to generate the 3D consolidated images. The principles of the 3D reconstruction is based on so called DFD (Depth From Defocus). In this process images are acquired and stacked while lifting the lens in the z direction. For each step the sharp part is considered and the no sharp part is disregarded. After the process of acquisition is carried out the complete z stack is reconstructed. As the steps of the Z axis are known as well as the x and y dimensions of a calibrated lens, a realistic 3D picture can be generated with realistic dimensions.
A 3D stack of images
3D acquisitions at tilted angles
If only the z-axis is moved, details which are positioned perpendicular towards the z can not be seen and hence not 3D reconstructed. The last couple of years technology has been developed using more sophisticated motorized stands which allow inspection of lateral sides.
An advanced motorized stand with 5 positioning vectors